Cross-matching blood

Blood for transfusion is cross-matched by incubating recipient serum with donor RBCs.

Direct antiglobulin test:
Agglutination → recipient has IgM against donor RBCs.

Indirect antiglobulin test (Indirect Coombs Test):
Adding human antiglobulin → agglutination → recipient has IgG against donor RBCs.

Robin Coombs, Cambridge, 1921–